Laboratory Applications of Furosemide
Furosemide is a potent loop diuretic belonging to the class of benzothiadiazine compounds, originally developed by Sanofi for clinical applications. This pharmaceutical-grade compound operates through specific inhibition of the sodium-potassium-chloride co-transporter in the ascending limb of Henle’s loop, making it valuable for researchers investigating renal physiology and fluid balance mechanisms. Laboratory studies frequently examine furosemide’s rapid onset kinetics and dose-dependent diuretic effects in various experimental models.
Research facilities investigating metabolic pathways often utilise furosemide to study fluid homeostasis, electrolyte transport, and cardiovascular responses. The compound’s well-characterised pharmacokinetic profile includes approximately 65% oral bioavailability with peak plasma concentrations occurring within 1-2 hours. Scientists at Stroid UK Shop can access pharmaceutical-grade furosemide for comprehensive laboratory investigations requiring consistent, reproducible results across experimental protocols.
Research Protocol Considerations
When designing experimental protocols, researchers must consider furosemide’s mechanism involves competitive inhibition of chloride binding sites on the NKCC2 transporter. This specificity allows investigators to examine discrete aspects of renal function without affecting other transport mechanisms. Studies examining metabolic modulators and diuretics frequently incorporate furosemide as a reference compound due to its predictable dose-response characteristics and extensive literature documentation.
Comparative studies often evaluate furosemide alongside other research compounds, including selective androgen receptor modulators or peptide compounds, to examine potential interactions or combined effects on fluid balance and metabolic parameters. Laboratory protocols typically investigate dosage ranges from 10-80mg equivalents in cellular and tissue models.
Compound Specifications
| Parameter | Specification |
|---|---|
| Chemical Name | 4-Chloro-N-furfuryl-5-sulfamoylanthranilic acid |
| CAS Registry | 54-31-9 |
| Molecular Formula | C₁₂H₁₁ClN₂O₅S |
| Molecular Weight | 330.74 g/mol |
| Manufacturer | Sanofi |
| Storage Requirements | Room temperature, protect from light and moisture |
Compliance Statement
This furosemide product is intended exclusively for in-vitro laboratory research applications. Not approved for human consumption, therapeutic use, or any application outside controlled laboratory environments. Researchers must ensure proper institutional approvals and safety protocols before conducting any studies involving this compound.
